The mechanisms underlying pluripotency and differentiation in embryonic and reprogrammed stem cells are unclear. In this\r\nwork, we characterized the pluripotent state towards neural differentiated state through analysis of trace elements\r\ndistribution using the Synchrotron Radiation X-ray Fluorescence Spectroscopy. Naive and neural-stimulated embryoid\r\nbodies (EB) derived from embryonic and induced pluripotent stem (ES and iPS) cells were irradiated with a spatial resolution\r\nof 20 mm to make elemental maps and qualitative chemical analyses. Results show that these embryo-like aggregates\r\nexhibit self-organization at the atomic level. Metallic elements content rises and consistent elemental polarization pattern of\r\nP and S in both mouse and human pluripotent stem cells were observed, indicating that neural differentiation and\r\nelemental polarization are strongly correlated.
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